| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LOVELL, Makenna | SO | Jacksonville (H.S.) | 34.04 | Verified |
| 2 | FREESEN, Sophia | 9 | Pittsfield | 33.23 | Unverified: |
| 3 | VANDIVER, Sophia | 9 | Hannibal High School | 31.90 | Verified |
| 4 | TOELKE, Katelyn | 9 | Pittsfield | 33.22 | Verified |
| 5 | HULL, McKenna | SO | Hannibal High School | 33.83 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ELEAZARRARAZ, Sofia | 9 | South Shelby High School | 31.48 | Verified |
| 2 | PALJAKKA-CARGO, Nora | 9 | Ft. Zumwalt South High School | 30.83 | Verified |
| 3 | GARNETT, Avaleigh | JR | Hannibal High School | 30.38 | Verified |
| 4 | DEAN, Samara | SR | Hannibal High School | 30.45 | Verified |
| 5 | GLAENZER, Naomi | SO | Jacksonville (H.S.) | 30.84 | Verified |
| 6 | JOHNSON, Nevaeh | 9 | Hannibal High School | 31.62 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SHEPHERD, Shaelyn | 9 | Mark Twain High School | 29.80 | Verified |
| 2 | CRANE, Kameil | SO | Hannibal High School | 29.60 | Verified |
| 3 | DOUGLAS, Ayanna | 9 | Quincy (Sr.) | 29.19 | Verified |
| 4 | BOLEACH, Madison | SO | Mark Twain High School | 29.27 | Verified |
| 5 | THOMAS, Maya | JR | Kirksville High School | 29.76 | Verified |
| 6 | JAMES, Rachel | SO | Carthage (Illini West) | 29.82 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FREY, Daisy | SO | Liberty | 28.90 | Verified |
| 2 | TURNER, Tanner | SR | Bowling Green High School | 28.30 | Verified |
| 3 | DOWNEN, Phebe | JR | Kirksville High School | 28.20 | Verified |
| 4 | LAJOY, Jaylianna | 9 | Hannibal High School | 28.23 | Unverified: |
| 5 | BARBAGIOVANNI, Alden | JR | Quincy (Sr.) | 28.79 | Unverified: |
| 6 | LOPEZ, Alixzandria | 9 | Carthage (Illini West) | 28.96 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DEAN, Sameya | 9 | Hannibal High School | 28.18 | Verified |
| 2 | WHITE, Taytum | SO | Palmyra High School | 27.12 | Verified |
| 3 | LEWIS, Kelley | SR | Bowling Green High School | 26.38 | Verified |
| 4 | SUNDE, Elliana | SR | Liberty | 26.90 | Verified |
| 5 | CLARK, Inaiah | SO | Ft. Zumwalt South High School | 28.01 | Verified |
| 6 | WILT, Madison | 9 | South Shelby High School | 28.19 | Verified |
