| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SHEPUTIS, Laurin | SO | Palmyra High School | 2:28.22 | Verified |
| 2 | NEISEN, Arianna | SO | Liberty | 2:31.40 | Unverified: |
| 3 | DORSEY, Jocelyn | SO | Hannibal High School | 2:32.13 | Verified |
| 4 | BRYAN, Josie | SO | Carthage (Illini West) | 2:32.51 | Verified |
| 5 | SCHUMACHER, Isabelle | JR | Ft. Zumwalt South High School | 2:36.01 | Verified |
| 6 | SMITH, Carlee | SO | Kirksville High School | 2:37.72 | Verified |
| 7 | WILLIAMS, Hannah | 9 | Pittsfield | 2:38.12 | Verified |
| 8 | DEJAYNES, Michelle | 9 | Pittsfield | 2:43.04 | Verified |
| 9 | SUMMERS, Bella | SO | Jacksonville (H.S.) | 2:44.00 | Unverified: |
| 10 | GOERLICH, Natalie | SR | Quincy (Sr.) | 2:46.37 | Verified |
| 11 | ELEAZARRARAZ, Sofia | 9 | South Shelby High School | 2:46.78 | Verified |
| 12 | ELLIS, Matera | JR | Mark Twain High School | 2:49.20 | Verified |
| 13 | HICKERSON, Natalie | 9 | Bowling Green High School | 2:49.48 | Verified |
| 14 | VOSS, Nicole | SR | Ft. Zumwalt South High School | 2:52.61 | Verified |
| 15 | DAVIS, Morgan | JR | Quincy (Sr.) | 2:55.40 | Verified |
| 16 | BIBB, Claire | 9 | Bowling Green High School | 2:58.93 | Verified |
| 17 | BOOTH, Lauren | JR | Mark Twain High School | 2:59.05 | Verified |
| 18 | CARROLL, Audra | 9 | Carthage (Illini West) | 3:06.01 | Unverified: |
| 19 | SPARROW, Karlie | SO | Liberty | 3:11.53 | Verified |
| 20 | WIGFALL, Riah | 9 | Hannibal High School | 3:12.32 | Verified |
| 21 | MELNICK, Tyne | 9 | Kirksville High School | 3:12.46 | Verified |
| 22 | ROSS, Raine | SR | Jacksonville (H.S.) | 3:20.00 | Unverified: |
