| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | COOK, Addison | 9 | Carthage (Illini West) | NT | Unverified: |
| 3 | WELLS, Natalee | SO | Bowling Green High School | 1:00.72 | Verified |
| 4 | ESPINOZA, Yoanny | SR | Carthage (Illini West) | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | KRIGBAUM, Emma | JR | Palmyra High School | 56.31 | Unverified: Clark County Relays |
| 2 | JOHNSON, Jayiona | 9 | Ft. Zumwalt South High School | 55.77 | Verified |
| 3 | MITCHELLE, Belle | SR | Liberty | 54.34 | Verified |
| 4 | SUMMERS, Kaley | 9 | Quincy (Sr.) | 55.30 | Unverified: |
| 5 | SPOONER, Eleanor | SR | Quincy (Sr.) | 56.30 | Unverified: |
| 6 | GIBBONS, Haley | SR | Jacksonville (H.S.) | 57.50 | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROWN, Riyeen | 9 | Bowling Green High School | 52.99 | Verified |
| 2 | LYONS, Dawsyn | JR | Kirksville High School | 52.30 | Verified |
| 3 | VOSS, Tenlee | JR | Palmyra High School | 50.72 | Verified |
| 4 | HARK, Nora | JR | Hannibal High School | 51.07 | Verified |
| 5 | THOMPSON, Jorie | SO | Hannibal High School | 52.46 | Verified |
| 6 | MILLER, Sydney | 9 | Mark Twain High School | 53.13 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DOUGLAS, Triniti | JR | Ft. Zumwalt South High School | 50.00 | Verified |
| 2 | TEN EYCK, Ellie | JR | Pittsfield | 49.16 | Unverified: |
| 3 | O'NEIL, Shannon | SR | Liberty | 48.37 | Verified |
| 4 | HERBST, Jersey | SO | Kirksville High School | 48.76 | Unverified: Gerald Mansfield Invitational |
| 5 | COX, Katie | SR | Pittsfield | 49.47 | Unverified: |
| 6 | MITCHELL, Kamryn | SO | South Shelby High School | 50.30 | Unverified: |
