| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Carthage (Illini West) | A | James | Shutwell | Hunter | Lambert | 58.99 | Verified |
| 3 | South Shelby High School | A | McWilliams | Gaines | Mitchell | Roush | 54.64 | Unverified: |
| 4 | Rushville (R.-Industry) | A | Ntumba | Wrench | Prather | Sewika | 55.79 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Pittsfield | A | Waters | Cox | Ten Eyck | Preist | 53.42 | Verified |
| 2 | Palmyra High School | A | Redd | Sublette | McBride | Juette | 52.49 | Verified |
| 3 | Ft. Zumwalt South High School | A | 51.00 | Unverified: | ||||
| 4 | Hannibal High School | A | Williams | Dean | Stewart | LaJoy | 52.42 | Verified |
| 5 | Quincy (Sr.) | A | McCoy | Hodges | Douglas | England | 53.23 | Unverified: |
| 6 | Mark Twain High School | A | Boleach | Armour | Black | Ross | 53.70 | Verified |
