| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROSEN, Fiker | SR | Quincy (Sr.) | 4:31.00 | Unverified: |
| 2 | WOLF, Cannen | SR | Liberty | 4:31.28 | Unverified: |
| 3 | MCCLELLAND, Eric | JR | Quincy (Sr.) | 4:37.00 | Unverified: |
| 4 | CHANCE, Ben | SO | Bowling Green High School | 4:39.90 | Verified |
| 5 | HESS, Eli | JR | Hannibal High School | 4:49.33 | Verified |
| 6 | WILT, Noah | 9 | South Shelby High School | 4:52.24 | Verified |
| 7 | JUETTE, Caleb | SR | Palmyra High School | 4:57.61 | Verified |
| 8 | BLAINE, Daytona | SR | South Shelby High School | 5:00.81 | Verified |
| 9 | BROSS, Connor | JR | Palmyra High School | 5:04.64 | Verified |
| 10 | MEYER, Alan | JR | Ft. Zumwalt North High School | 5:06.64 | Verified |
| 11 | MUNGER, David | 9 | Hannibal High School | 5:07.37 | Verified |
| 12 | VEILE, Brayden | JR | Pittsfield | 5:09.20 | Unverified: |
| 13 | MARGERUM, Blake | SO | Ft. Zumwalt North High School | 5:10.41 | Verified |
| 14 | OLSON, Vinny | SO | Pittsfield | 5:15.06 | Verified |
| 15 | BLACK, Brandon | JR | Mark Twain High School | 5:15.57 | Verified |
| 16 | HENNINGFIELD, Elija | 9 | Bowling Green High School | 5:15.72 | Verified |
| 17 | MARLIN, Jack | SR | Kirksville High School | 5:18.51 | Verified |
| 18 | KOCH, Dylan | SO | Rushville (R.-Industry) | 5:26.50 | Verified |
| 19 | FUNK, Devon | SO | Liberty | 5:27.95 | Verified |
| 20 | ACKMAN, Miles | JR | Rushville (R.-Industry) | 5:31.43 | Verified |
| 21 | GRAY, Micah | SR | Ft. Zumwalt South High School | 5:31.53 | Verified |
| 22 | MCGRANE, Dennis | SR | Ft. Zumwalt South High School | 5:42.14 | Verified |
| 23 | MCAULIFFE, Spencer | SR | Jacksonville (H.S.) | 5:54.40 | Verified |
| 24 | SOLTERMANN, Andrew | 9 | Jacksonville (H.S.) | 5:54.85 | Verified |
| 25 | BROWN, Spencer | JR | Carthage (Illini West) | 6:29.61 | Verified |
| 26 | MILLER, Coden | 9 | Mark Twain High School | NT | Unverified: |
