| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palmyra High School | A | Obrien | Noland | Loman | Sheputis | 10:23.13 | Verified |
| 2 | Ft. Zumwalt South High School | A | 10:54.69 | Verified | ||||
| 3 | South Shelby High School | A | Mitchell | McWilliams | Hunolt | Roush | 10:57.59 | Verified |
| 4 | Bowling Green High School | A | Mcmillen | Hickerson | Waddell | Bibb | 11:19.76 | Verified |
| 5 | Mark Twain High School | A | Ellis | Northcutt | Resor | Miller | 11:24.89 | Verified |
| 6 | Quincy (Sr.) | A | Goerlich | Schneider | Bange | Davis | 11:25.00 | Unverified: |
| 7 | Carthage (Illini West) | A | Whitaker | Cook | Ray | Bryan | 11:29.36 | Unverified: |
| 8 | Hannibal High School | A | Griffen | Waelder | Bonvillian | Bates | 11:34.96 | Verified |
| 9 | Jacksonville (H.S.) | A | Wyman | Cunningham | Zoellner | Ross | 11:45.07 | Verified |
| 10 | Kirksville High School | A | McNeely | Smith | Starkey | Sterup | 12:07.74 | Verified |
| 11 | Rushville (R.-Industry) | A | Armstrong | Haines | Park | Lyons | 13:57.30 | Verified |
