| PL | Athlete | Yr. | Team | Time | Pts | HPL |
|---|---|---|---|---|---|---|
| 1 | SUNDE, Elliana | SR | Liberty | 27.08 | 10 | 5 (1) |
| 2 | LEWIS, Kelley | SR | Bowling Green High School | 27.45 | 8 | 5 (2) |
| 3 | WHITE, Taytum | SO | Palmyra High School | 27.64 | 6 | 5 (3) |
| 4 | CLARK, Inaiah | SO | Ft. Zumwalt South High School | 27.68 | 4 | 5 (4) |
| 5 | LAJOY, Jaylianna | 9 | Hannibal High School | 28.08 | 2 | 4 (1) |
| 6 | DEAN, Sameya | 9 | Hannibal High School | 28.21 | 1 | 5 (5) |
| 7 | FREY, Daisy | SO | Liberty | 28.22 | 4 (2) | |
| 8 | BARBAGIOVANNI, Alden | JR | Quincy (Sr.) | 28.74 | 4 (3) | |
| 9 | TURNER, Tanner | SR | Bowling Green High School | 28.97 | 4 (4) | |
| 10 | THOMAS, Maya | JR | Kirksville High School | 29.18 | 3 (1) | |
| 11 | CRANE, Kameil | SO | Hannibal High School | 29.57 | 3 (2) | |
| 12 | DOUGLAS, Ayanna | 9 | Quincy (Sr.) | 29.72 | 3 (3) | |
| 13 | LOPEZ, Alixzandria | 9 | Carthage (Illini West) | 29.77 | 4 (5) | |
| 14 | SHEPHERD, Shaelyn | 9 | Mark Twain High School | 29.89 | 3 (4) | |
| 15 | DEAN, Samara | SR | Hannibal High School | 29.90 | 2 (1) | |
| 16 | GARNETT, Avaleigh | JR | Hannibal High School | 30.02 | 2 (2) | |
| 17 | GLAENZER, Naomi | SO | Jacksonville (H.S.) | 30.04 | 2 (3) | |
| 18 | JAMES, Rachel | SO | Carthage (Illini West) | 30.81 | 3 (5) | |
| 19 | JOHNSON, Nevaeh | 9 | Hannibal High School | 31.20 | 2 (4) | |
| 20 | VANDIVER, Sophia | 9 | Hannibal High School | 31.69 | 1 (1) | |
| 21 | HULL, McKenna | SO | Hannibal High School | 33.33 | 1 (2) | |
| 22 | TOELKE, Katelyn | 9 | Pittsfield | 33.53 | 1 (3) | |
| 23 | FREESEN, Sophia | 9 | Pittsfield | 33.90 | 1 (4) | |
| BOLEACH, Madison | SO | Mark Twain High School | NT | |||
| PALJAKKA-CARGO, Nora | 9 | Ft. Zumwalt South High School | NT | |||
| ELEAZARRARAZ, Sofia | 9 | South Shelby High School | NT | |||
| LOVELL, Makenna | SO | Jacksonville (H.S.) | NT | |||
| DOWNEN, Phebe | JR | Kirksville High School | NT | |||
| WILT, Madison | 9 | South Shelby High School | NT |
