| PL | Athlete | Yr. | Team | Time | Pts | HPL |
|---|---|---|---|---|---|---|
| 1 | TEN EYCK, Ellie | JR | Pittsfield | 47.98 | 10 | 4 (1) |
| 2 | O'NEIL, Shannon | SR | Liberty | 48.28 | 8 | 4 (2) |
| 3 | HERBST, Jersey | SO | Kirksville High School | 49.47 | 6 | 4 (3) |
| 4 | MITCHELL, Kamryn | SO | South Shelby High School | 50.95 | 4 | 4 (4) |
| 5 | LYONS, Dawsyn | JR | Kirksville High School | 51.47 | 2 | 3 (1) |
| 6 | JOHNSON, Jayiona | 9 | Ft. Zumwalt South High School | 51.98 | 1 | 2 (1) |
| 7 | HARK, Nora | JR | Hannibal High School | 52.24 | 3 (2) | |
| 8 | BROWN, Riyeen | 9 | Bowling Green High School | 53.78 | 3 (3) | |
| 9 | MILLER, Sydney | 9 | Mark Twain High School | 54.04 | 3 (4) | |
| 10 | SUMMERS, Kaley | 9 | Quincy (Sr.) | 55.67 | 2 (2) | |
| 11 | KRIGBAUM, Emma | JR | Palmyra High School | 57.96 | 2 (3) | |
| 12 | MITCHELLE, Belle | SR | Liberty | 59.28 | 2 (4) | |
| 13 | SPOONER, Eleanor | SR | Quincy (Sr.) | 59.89 | 2 (5) | |
| 14 | ESPINOZA, Yoanny | SR | Carthage (Illini West) | 1:00.42 | 1 (1) | |
| 15 | WELLS, Natalee | SO | Bowling Green High School | 1:01.83 | 1 (2) | |
| 16 | COOK, Addison | 9 | Carthage (Illini West) | 1:02.41 | 1 (3) | |
| THOMPSON, Jorie | SO | Hannibal High School | DQ | |||
| GIBBONS, Haley | SR | Jacksonville (H.S.) | NT | |||
| VOSS, Tenlee | JR | Palmyra High School | NT | |||
| COX, Katie | SR | Pittsfield | NT | |||
| DOUGLAS, Triniti | JR | Ft. Zumwalt South High School | NT |
