| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Southern Boone County High Schoo | A | Lutz | Dial | Bishop | High | 1:39.43 | Verified |
| 4 | Capital City High School | A | Obi | Hankins | Bonchonsky | Huggins | 1:32.25 | Verified |
| 5 | Battle High School | A | Weir | Rice | Adam | Anderson | 1:38.19 | Verified |
