| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LOGAN, Kamiya | SR | Battle High School | NT | Unverified: |
| 2 | NISHIMWE, Deborah | 9 | Battle High School | NT | Unverified: |
| 3 | WEISS, Linea | SR | Battle High School | 1:26.92 | Verified |
| 4 | MORENO, Karina | 9 | Capital City High School | 1:21.59 | Verified |
| 5 | COLBURN, Ellie | 9 | Hickman High School | 1:24.87 | Verified |
| 6 | HIGHTOWER, Aniyah | 9 | Capital City High School | 1:40.47 | Verified |
| 7 | OPARA, Cristal | 9 | Capital City High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LONG, Julianne | 9 | Southern Boone County High Schoo | 1:15.89 | Unverified: |
| 2 | CONCANNON, Sophia | JR | Rock Bridge High School | 1:12.08 | Unverified: |
| 3 | HAYES, Melora | 9 | Hickman High School | 1:10.70 | Verified |
| 4 | PETERSON, Athena | 9 | Hickman High School | 1:04.00 | Unverified: |
| 5 | DOMEIER, Isabelle | 9 | Hickman High School | 1:08.53 | Verified |
| 6 | NUSBAUM, Eden | 9 | Hickman High School | 1:11.47 | Verified |
| 7 | GOOCH, Emma | SO | Hickman High School | 1:14.52 | Verified |
| 8 | BRASE, Kalley | 9 | Capital City High School | 1:16.50 | Verified |
