| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Battle High School | A | Adam | Carter | Love | Weir | 45.70 | Verified |
| 5 | Capital City High School | A | Samuel | McNeil | Barnes | Otten | 48.00 | Unverified: |
| 6 | Southern Boone County High Schoo | A | Lutz | Bishop | High | Dial | 48.21 | Verified |
| 7 | Rock Bridge High School | A | Rychnovsky | Chase | Rolwing | Smith | 48.20 | Unverified: |
