| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rock Bridge High School | A | Dawson | Scott | Lockett | McCall | 56.00 | Unverified: |
| 2 | Capital City High School | A | Opara | Borde-Koufie | Kamara | York | 53.74 | Verified |
| 3 | Battle High School | A | May | Hopkins | Schultz | Carter | 55.60 | Verified |
