| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bishop DuBourg High School | A | BROWN | Olayo | PARKER | Reynolds | NT | Unverified: |
| 4 | Villa Duchesne High School | A | Wilson | Mosby | Procell | Harper | 1:05.19 | Verified |
| 5 | St. Joseph's Academy | A | Bittle | Scheibelhut | Dignam | Hendricks | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Ignatius College Prep High S | A | 59.94 | Verified | ||||
| 2 | Visitation Academy | A | 58.90 | Verified | ||||
| 3 | Althoff Catholic High School | A | Cange | Gordon | Hebel | Conrad | 55.21 | Verified |
| 4 | Helias Catholic High School | A | Phillips | Struemph | Harrison | Edwards | 52.12 | Verified |
| 5 | Cor Jesu Academy | A | Varghese | Stoeckel | Lorms | Eilerman | 54.46 | Unverified: |
| 6 | Rosati-Kain High School | A | Hemphill | Turner | Johnson | Elston | 57.24 | Verified |
| 7 | Ursuline Academy | A | 59.33 | Verified | ||||
| 8 | Notre Dame (St. Louis) High Scho | A | 1:04.14 | Verified |
