| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | TATKO, Anna | SO | Bishop DuBourg High School | 1:02.21 | Verified |
| 3 | ELSTON, Brooke | JR | Rosati-Kain High School | 56.28 | Verified |
| 4 | KUNZ, Kelsey | 9 | St. Dominic High School | 54.98 | Verified |
| 5 | SIEBERT, Lydia | 9 | Father Tolton Regional Catholic | 55.79 | Verified |
| 6 | KASSEBAUM, Raquel | SR | Christian High School | 59.64 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | RAITHEL, Alexis | SO | Lutheran (St. Charles) High Scho | 51.90 | Verified |
| 3 | REYNOLDS, Ellie | SR | Father Tolton Regional Catholic | 48.47 | Verified |
| 4 | TAYLOR, alexis | JR | Cardinal Ritter High School | 47.07 | Verified |
| 5 | BRYANT, Neveah | 9 | Cardinal Ritter High School | 47.65 | Unverified: |
| 6 | WAGNER, Chloe | JR | Christian High School | 50.63 | Verified |
| 7 | TRIMBLE, Chloe | JR | Lutheran (St. Charles) High Scho | 52.35 | Verified |
