| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Bishop DuBourg High School | A | Becton | DAVIS | Floyd | GARDNER | 48.60 | Verified |
| 4 | Christian High School | A | Connell | Schroer | Evans | Tucker | 46.62 | Verified |
| 5 | Father Tolton Regional Catholic | A | Baggett | Hargis | Jacaway | Willmeth | 47.40 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Dominic High School | A | Phillips | Livers | Powell | Powell | DQ | Verified |
| 3 | Lutheran (St. Charles) High Scho | A | 43.95 | Verified | ||||
| 4 | Cardinal Ritter High School | A | 43.80 | Verified | ||||
| 5 | St. Mary's (St. Louis) High Scho | A | Roberts | Washington | Hendricks | McIntosh | 43.91 | Verified |
| 6 | Duchesne High School | A | Jarriett | Jones | Oliphant | Longley | 45.53 | Verified |
| 7 | St. Francis Borgia High School | A | Zeltmann | Mitchell | Volmert | Mohesky | 46.30 | Verified |
