| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clopton Middle School | A | Hall | Salmons | Alexander | Eivins | 2:39.15 | Verified |
| 2 | St. Louis Blue Knights Middle Sc | B | Stiles | Koenig | Lily | Barton | 2:16.38 | Verified |
| 3 | Elsberry Middle School | A | Toland | Heras | Santos | Underwood | 2:01.89 | Verified |
| 4 | Wright City MS | A | Loeffler | Cross | McGowan | Stufflebean | 1:48.71 | Verified |
| 5 | Winfield MS | A | Burk | Johnson | Dillin | Colbert | 1:52.84 | Verified |
| 6 | Black Hawk Middle School | A | Beckemeyer | Grooms | Mcclearey | Forrest | 2:10.60 | Verified |
| 7 | St. Louis Blue Knights Middle Sc | A | Barfield | Snell | Clipper | Criswell | 2:16.38 | Verified |
