| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Louis Blue Knights Middle Sc | B | Paramathas | Riggs | Tallo | Velius | 2:28.39 | Verified |
| 2 | Black Hawk Middle School | B | Sharp | Geurin | Allen | Duncan | 2:13.71 | Verified |
| 3 | Wright City MS | A | Palmer | Rees | Head | Brown | 2:11.58 | Verified |
| 4 | Elsberry Middle School | A | Talbot | Howard | Hartley | Talbot | 1:59.78 | Verified |
| 5 | Winfield MS | A | Liberson | Pund | Rau | Ford | 2:07.55 | Verified |
| 6 | Black Hawk Middle School | A | Marschel | Lombardo | Jackson | Morris | 2:13.71 | Verified |
| 7 | Clopton Middle School | A | Williamson | Bankhead | Graham | Mitchell | 2:19.06 | Verified |
| 8 | St. Louis Blue Knights Middle Sc | A | Riggs | Mullinnax | Barton | Szymanski | 2:28.39 | Verified |
