| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Louisiana Middle School | B | Jenkins | Anderson | Miller | Perrine | NT | Unverified: |
| 2 | Wright City MS | B | Dalton | Nunez-Cruz | Plunkett | Grueneberg | NT | Unverified: |
| 3 | Bowling Green Middle School | A | Bibb | Bibb | Hickerson | Hickerson | NT | Unverified: |
| 4 | Bowling Green Middle School | B | Kent | Haywood | Hartwig | Brewster | NT | Unverified: |
| 5 | Silex Middle School | A | James | Mehler | Claborn | Burks | NT | Unverified: |
| 6 | Louisiana Middle School | A | Gomez | Sperry | Chatman | Bradley | NT | Unverified: |
| 7 | Winfield MS | A | Duncan | Lasswell | Meyer | Puder | NT | Unverified: |
| 8 | Wright City MS | A | Kirn | Love | Noltkamper | Ritter | NT | Unverified: |
