| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Winfield MS | B | Penrod | Dillin | Johnson | Burk | NT | Unverified: |
| 2 | Wright City MS | A | Boeckman | Boeckman | Loeffler | Bennett | NT | Unverified: |
| 3 | Winfield MS | A | Morse | Moore | Martin | Barth | NT | Unverified: |
| 4 | Elsberry Middle School | A | Byers | Crenshaw | Edwards | Heras | 1:02.72 | Verified |
| 5 | Louisiana Middle School | B | Mayfield | Ulloa | Brown | Wilkinson | NT | Unverified: |
| 6 | Bowling Green Middle School | A | Lubrecht | Kearney | Horton | Buffington | NT | Unverified: |
| 7 | Louisiana Middle School | A | Barber | Mendez | Sheppard | Hathaway | NT | Unverified: |
