| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | REID, Lauren | SR | Palmyra High School | 5:59.28 | Verified |
| 2 | SZARKA, Lydia | SR | Palmyra High School | 6:05.73 | Verified |
| 3 | KLOTT, Rachel | 9 | Bowling Green High School | 6:10.00 | Unverified: Bowling Green Invitational |
| 4 | SNELL, Anna | JR | St. Louis Blue Knights | 6:42.69 | Verified |
| 5 | WADDELL, Lillian | JR | Bowling Green High School | 7:29.00 | Unverified: |
| 6 | BOSCHERT, Lily | SO | Winfield High School | 10:00.00 | Verified |
| 7 | RUDAR, Jessi | SO | Winfield High School | 10:00.00 | Verified |
| 8 | GRESHAM, Hannah | 9 | St. Louis Blue Knights | NT | Unverified: |
| 9 | WEITKAMP, Macy | JR | Clopton High School | NT | Unverified: |
| 10 | BERRY, Kade | SO | Winfield High School | NT | Unverified: |
| 11 | SNELL, Natalie | SR | St. Louis Blue Knights | NT | Unverified: |
| 12 | BLANK, Aubrey | SR | St. Louis Blue Knights | NT | Unverified: |
