| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SNELL, Jacob | SR | St. Louis Blue Knights | 4:53.26 | Verified |
| 2 | HEARN, Colton | JR | Bowling Green High School | 5:00.00 | Unverified: Bowling Green Invitational |
| 3 | CHANCE, Benjamin | 9 | Bowling Green High School | 5:01.25 | Unverified: |
| 4 | GRIFFITH, Trenton | SO | Bowling Green High School | 5:39.48 | Verified |
| 5 | JUETTE, Caleb | JR | Palmyra High School | 5:59.32 | Verified |
| 6 | MITTS, Zackary | SR | Winfield High School | 10:00.00 | Verified |
| 7 | BURK, Micah | 9 | Winfield High School | 10:00.00 | Verified |
| 8 | BROSS, Connor | SO | Palmyra High School | 10:00.00 | Verified |
| 9 | JANES, Jackson | SO | Palmyra High School | 10:00.00 | Verified |
| 10 | GRESHAM, Adam | JR | St. Louis Blue Knights | NT | Unverified: |
| 11 | PALUMBO, Nathan | JR | St. Louis Blue Knights | NT | Unverified: |
| 12 | MEYERS, Jacob | JR | St. Louis Blue Knights | NT | Unverified: |
