| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Webster Groves High School | A | Casseus | Fischer | Cooper | Krueger | NT | Verified |
| 3 | Ritenour High School | A | Weissler | Robinson | Kadima | Williams | 3:30.00 | Unverified: |
| 4 | Marquette High School | A | Bush | Burke | Brown | CODY-JACKSON | 3:36.15 | Verified |
| 5 | Parkway West High School | A | Hathaway | Kallaos | Muradyan | Fuller | 3:57.30 | Verified |
