| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Marquette High School | A | CLARK | EHIMARE | FLEMING | Tanzola | 5:02.40 | Verified |
| 3 | Webster Groves High School | A | King | Garrison | Murdock | Lemley | 4:31.55 | Verified |
| 4 | Parkway West High School | A | Whitaker | Plass | Drake | Woodall | 4:48.74 | Verified |
