| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marquette High School | A | Bush | Altamirano | James | Thoele | 8:24.02 | Verified |
| 2 | Webster Groves High School | A | Bacon | Krueger | Glarner | Lack | 8:39.52 | Verified |
| 3 | Parkway West High School | A | Hathaway | Kallaos | Muradyan | Fuller | 8:49.46 | Verified |
