| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WHITAKER, Kennedy | SO | Parkway West High School | 2:32.82 | Verified |
| 2 | STAAB, Sarah | 9 | Webster Groves High School | 2:33.57 | Unverified: |
| 3 | WELCH, Nora | JR | Webster Groves High School | 2:36.31 | Unverified: |
| 4 | ETCHASON, Alexandra | SO | Parkway West High School | 2:36.41 | Verified |
| 5 | HITE, Carlen | JR | Marquette High School | 2:47.80 | Verified |
| 6 | ARNOLD, Winne | SR | Marquette High School | 2:49.00 | Unverified: |
