| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Webster Groves High School | A | Bronner | Jackson | Henderson | Casseus | 48.13 | Verified |
| 3 | Ritenour High School | A | Kadima | Mitchell-Williams | Robinson | Moore | 45.00 | Unverified: |
| 4 | Marquette High School | A | Skinner | Jones | CODY-JACKSON | Kreh | 46.04 | Verified |
