| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SIPP, Emily | SR | Parkway West High School | 5:35.87 | Verified |
| 2 | STAAB, Sarah | 9 | Webster Groves High School | 6:01.47 | Verified |
| 3 | WELCH, Nora | JR | Webster Groves High School | 6:18.39 | Unverified: |
| 4 | GORING, SKYLAR | SO | Marquette High School | 6:25.80 | Unverified: |
| 5 | BARTON, Madeline | SO | Marquette High School | 6:29.00 | Unverified: |
| 6 | GUTIERREZ GAONA, Adriana | SO | Ritenour High School | 7:23.71 | Verified |
| 7 | WHITAKER, Kennedy | SO | Parkway West High School | NT | Unverified: |
