Section 1 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vianney High School | A | 52.34 | Verified | ||||
| 3 | Webster Groves High School | A | Maupin | Kaanagbara | Logan | Miller | 49.44 | Unverified: |
| 4 | Mehlville High School | A | Wolf | Silies | Ordaz | Londal | 51.20 | Unverified: Cape Invitational |
Section 2 of 2
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lindbergh High School | A | Balbo | Earley | Birk | Deterding | 49.29 | Verified |
| 2 | St. Louis University High School | A | Bailey | Chaffee | Salcedo | Verzino | 47.74 | Unverified: |
| 3 | Ladue Horton Watkins High School | A | Lee | Lucas | Fidler | Perry | 46.36 | Verified |
| 4 | Lafayette (Wildwood) High School | A | TINSLEY | Retych | VEHIGE | Cochran | 47.64 | Unverified: |
| 5 | Kirkwood High School | A | McCulley | Smith | Malone | Welch | 48.00 | Unverified: |
