| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ursuline Academy | A | 2:04.53 | Verified | ||||
| 4 | Visitation Academy | A | Slade | Sekhar | Gerty | Martin | 2:00.00 | Unverified: 2:00 |
| 5 | Villa Duchesne High School | A | Weingart | Minner | Schneithorst | Sykes | 2:00.62 | Verified |
