| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Ursuline Academy | A | 1:00.86 | Verified | ||||
| 4 | Visitation Academy | A | Van Vooren | Mank | Atkinson | McLellan | 56.00 | Unverified: 56.0 |
| 5 | Villa Duchesne High School | A | Hughes | Irvin | Wilson | Acker | 57.82 | Verified |
