| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wright City MS | A | Kirn | Love | Noltkamper | Palmer | NT | Verified |
| 3 | Black Hawk Middle School | A | Marschel | Morris | Donovan | Shaw | 4:46.25 | Verified |
| 4 | Hannibal Middle School | A | Groth | Stolte | Turner | Dean | 4:38.14 | Verified |
| 5 | Troy South MS | A | Getz | Haynes | Graf | Robinson | 4:46.03 | Verified |
| 6 | Wentzville South Middle School | A | Edwards | Climer | Martin | Papez | 4:58.79 | Unverified: Wentzville Middle Invitational |
