| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARSCHEL, Morgan | 8 | Black Hawk Middle School | 2:41.80 | Verified |
| 2 | KALBAC, Jillian | 8 | Frontier Middle School | 2:52.95 | Unverified: |
| 3 | GILBERT, Brooke | 7 | Troy South MS | 2:55.70 | Verified |
| 4 | SCHENE, Addyson | 8 | Wentzville South Middle School | 2:58.32 | Verified |
| 5 | BATES, Emilia | 8 | Hannibal Middle School | 3:00.19 | Verified |
| 6 | PAPEZ, Hannah | 8 | Wentzville South Middle School | 3:05.11 | Unverified: Wentzville Middle Invitational |
| 7 | FARAH, Natalie | 8 | Hannibal Middle School | 3:10.00 | Unverified: |
| 8 | FINKLANG, Andrea | 8 | Frontier Middle School | 3:12.60 | Verified |
| 9 | GRANA, Victoria | 8 | Frontier Middle School | 3:16.52 | Unverified: |
| 10 | HOOD, Samantha | 7 | Troy South MS | 3:51.32 | Verified |
| 11 | JOSE, Kim | 8 | Troy Middle School | 4:08.14 | Verified |
| 12 | SWOFFORD, Lylian | 8 | Black Hawk Middle School | NT | Unverified: |
| 13 | ARNOLD, Emily | 7 | Troy Middle School | NT | Unverified: |
| 14 | STANLEY, Lucy | 8 | Wentzville South Middle School | NT | Unverified: |
