| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Troy Middle School | A | Clausner | Culbertson | Eulinger | Lindsay | 52.65 | Verified |
| 3 | Hannibal Middle School | A | Closser | Jaworski | Odom | Bock | 51.67 | Verified |
| 4 | Wright City MS | A | Boeckman | Boeckman | Bennett | Cross | 47.49 | Unverified: Wright City Middle School Invita |
| 5 | Frontier Middle School | A | Orf | Fisher | Treadway | Scrogin | NT | Verified |
| 6 | Troy South MS | A | Rudert | Crawmer | Hastings | Mains | DQ | Verified |
| 7 | Black Hawk Middle School | A | Beckemeyer | Forrest | Fulton | Grooms | 55.11 | Verified |
