| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Francis Borgia High School | A | Mace | Mort | Lober | Lebish | 4:24.53 | Verified |
| 3 | Hannibal High School | A | Nichols | Tweedy | Clubine | Bergheger | 3:49.46 | Verified |
| 4 | Ft. Zumwalt South High School | A | Johnson | Bates | Clark | Moore | 3:37.15 | Verified |
| 5 | Troy Buchanan High School | A | Love | Ballard | Hogan | Benhardt | 3:39.00 | Verified |
| 6 | St. Charles High School | A | Maples | Bland | Walker | Warner | 3:51.25 | Verified |
| 7 | St. Charles West High School | A | Southard | Litton | Kudaravalli | Mace | NT | Unverified: |
