| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Charles High School | A | Oellermann | Starks | Rayburn | Gilkison | 4:44.62 | Verified |
| 3 | Troy Buchanan High School | A | Brown | Hemmer | Hunolt | Robinson | NT | Verified |
| 4 | St. Charles West High School | A | Myers | Myers | Zeier | Jackson | 4:25.83 | Verified |
| 5 | Ft. Zumwalt South High School | A | Burnett | Hirsch | Morrison | Whitten | 4:27.13 | Verified |
| 6 | Hannibal High School | A | Dorsey | Scott | Crane | Griffen | 4:34.15 | Verified |
| 7 | Incarnate Word Academy | A | Ross | Toal | Ross | Toal | NT | Unverified: |
