| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Westminster Christian Academy | A | McGowan | Buford | Bradley | Scheulen | 4:35.70 | Verified |
| 4 | Ft. Zumwalt South High School | A | Hart | Williams-harris | Holman | Whitten | 4:24.59 | Verified |
| 5 | Ft. Zumwalt West High School | A | Rider | Rowland | St. Jean | Mueller | 4:31.37 | Verified |
| 6 | Louisiana High School | A | Norvell | Campbell | Jenkins | Murray | 4:46.84 | Verified |
