| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | NASH, Taylor | 9 | St. Charles High School | NT | Unverified: |
| 2 | WITHERSPOON, Bria | SO | Westminster Christian Academy | 1:02.96 | Verified |
| 3 | ANDERSON, Satchal | SO | Ft. Zumwalt West High School | 1:00.35 | Verified |
| 4 | SPANN, Carlie | SO | Ft. Zumwalt East High School | 53.72 | Verified |
| 5 | ROHDE, Isabel | JR | Troy Buchanan High School | 55.35 | Verified |
| 6 | KEATTS, Kinslee | JR | Holt High School | 1:00.38 | Verified |
| 7 | SEVERNS, Katherine | 9 | Ft. Zumwalt West High School | 1:11.23 | Verified |
