| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Louisiana High School | A | Campbell | Chatman | Jenkins | Murray | 56.34 | Verified |
| 3 | St. Charles High School | A | Long | Nash | Hollrah | Cortes-Lopez | 54.64 | Verified |
| 4 | Ft. Zumwalt South High School | A | Chatman | Martin | Raife | Bass | 52.71 | Verified |
| 5 | Westminster Christian Academy | A | Bynum | Howard | White | Scheulen | 54.62 | Verified |
| 6 | Ft. Zumwalt West High School | A | Moresi | Walden | Mueller | Loggins | 54.95 | Verified |
