| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Westminster Christian Academy | A | Calmese | Cook | Farquharson | Howard | 1:38.31 | Verified |
| 3 | Ft. Zumwalt South High School | A | Pryor | Paskach | Schaefering | Pordos | NT | Verified |
| 4 | Holt High School | A | Bunch | Hicks | Macklin | Merrell | 1:31.40 | Verified |
| 5 | Troy Buchanan High School | A | LaRue | Bellah | Best | Keene | 1:32.30 | Verified |
| 6 | St. Charles West High School | A | Patel | Rycraw | Tsahiridis | Williams | NT | Verified |
| 7 | St. Charles High School | A | Richard | Miller | Hayes | Calmese | 1:41.72 | Verified |
