| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Holt High School | A | Mosher | Pollock | Manion | Yang | 2:12.65 | Verified |
| 4 | Ft. Zumwalt South High School | A | Chatman | Martin | Raife | Bass | 1:50.49 | Verified |
| 5 | Ft. Zumwalt West High School | A | Moresi | St. Jean | Walden | Loggins | 1:55.17 | Verified |
