| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Ft. Zumwalt West High School | A | Orr | Snyder | Hammond | Kidnie | 8:10.17 | Verified |
| 2 | St. Charles West High School | A | Leach | Severs | Stockman | Guerrero | 8:33.48 | Verified |
| 3 | St. Charles High School | A | Beskorovany | Adams | Wirt | Suraty | NT | Verified |
| 4 | Ft. Zumwalt South High School | A | Walker | Moore | Gray | Mcgrane | 8:59.01 | Verified |
