| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WOOTEN, Karlie | Ultimate Speed Academy | 2:21.38 | ||
| 2 | BOLDEN, Ariel | Ultimate Speed Academy | 2:36.09 | ||
| 3 | REED, Kennedie | Ultimate Speed Academy | 2:36.42 | ||
| 4 | FITZPATRICK, Symia | Unattached | 2:40.06 | ||
| 5 | BOOKER, Kristen | Blues Track Club of Greater St | NT |
