| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lutheran South High School | A | Barnett | Pittman | Truong | Truong | NT | Unverified: |
| 3 | St. Louis Blue Knights | A | Ward | Rodgers | Twohy | Barfield | 1:58.40 | Verified |
| 4 | MICDS | A | Onovwerosuoke | Brown | Moore | Nunn | 1:34.94 | Verified |
| 5 | John Burroughs High School | A | 1:37.00 | Unverified: | ||||
| 6 | Principia High School | A | Asinga | Gathungu | Jordan | Rodriguez | NT | Unverified: |
