| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lutheran South High School | A | Barnett | Cousins | Kinder | Pittman | NT | Unverified: |
| 3 | John Burroughs High School | A | NT | Unverified: | ||||
| 4 | MICDS | A | Onovwerosuoke | Nunn | Moore | Putman | 3:43.65 | Unverified: |
| 5 | St. Louis Blue Knights | A | Schark | Majors | Hillebrand | Snell | 4:02.39 | Verified |
| 6 | Principia High School | A | Asinga | Omware | Rodriguez | Sempubu | NT | Unverified: |
