| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | MICDS | A | Byrne | Helms | Strelo | Helms | 9:25.40 | Unverified: |
| 2 | St. Louis Blue Knights | A | Barfield | Lily | Ward | Meyers | 9:49.20 | Verified |
| 3 | Principia High School | A | Farson | Omware | Proctor | Nutwell | NT | Unverified: |
| 4 | John Burroughs High School | A | NT | Unverified: | ||||
| 5 | Clayton High School | A | Lagesse | Lenze | Mysorekar | Patel | NT | Unverified: |
| 6 | Lutheran South High School | A | NT | Unverified: |
