| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Principia High School | A | NT | |||||
| 2 | Clayton High School | A | Cheng | Boyd | Simmons | Erdmann | NT | Unverified: |
| 3 | John Burroughs High School | A | NT | Unverified: | ||||
| 4 | MICDS | A | Gira | Anderson | Bellamy | Goot | 4:01.72 | Unverified: |
| 5 | St. Louis Blue Knights | A | Bryant | Majors | Snell | Snell | 4:48.65 | Verified |
| 6 | Lutheran South High School | A | Saitz | Hayes | Kramer | Allen | NT | Unverified: |
