| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Principia High School | A | Rodriguez | Sempubu | Farson | Omware | NT | Unverified: |
| 3 | Lutheran South High School | A | Landau | Kinder | Burgdorf | Ward | NT | Unverified: |
| 4 | MICDS | A | Onovwerosuoke | Brown | Nunn | Byrne | 4:03.26 | Unverified: |
| 5 | Clayton High School | A | Pickett | Snyder | Gruen | Snyder | NT | Unverified: |
| 6 | John Burroughs High School | A | NT | Unverified: | ||||
| 7 | St. Louis Blue Knights | A | Drago | Majors | Schark | Snell | NT | Unverified: |
