| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lutheran South High School | A | Meyer | Kramer | Watson | Allen | NT | Unverified: |
| 2 | Principia High School | A | Dow | Nutwell | Thomas | Hinchman | NT | Unverified: |
| 3 | St. Louis Blue Knights | A | Snell | Majors | Bryant | Snell | DQ | Unverified: |
| 4 | MICDS | A | Harrell | Ferguson | Gira | Williamson | 4:55.51 | Unverified: |
| 5 | Miller Career Academy | A | Ammons | Harris | McLaurin | RUTLIN | NT | Unverified: |
| 6 | Clayton High School | A | Wirthlin | Furdek | Osborne | Dayal | NT | Unverified: |
| 7 | John Burroughs High School | A | NT | Unverified: |
