| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Lutheran South High School | A | Cousins | Helt | Samking | Pittman | NT | Unverified: |
| 3 | St. Louis Blue Knights | A | Ward | Rodgers | Twohy | Larson | 55.14 | Verified |
| 4 | MICDS | A | Putman | Moore | Hall | Sutton | 45.55 | Verified |
| 5 | John Burroughs High School | A | 46.80 | Unverified: | ||||
| 6 | Principia High School | A | Asinga | Rodriguez | Sempubu | Vucinovich | DNS | Unverified: |
