| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Metro High School | A | Baum-Scholle | Cade | Christian | Kovarik | NT | Unverified: |
| 4 | STEAM Academy at McCluer South-B | A | 3:58.00 | Unverified: | ||||
| 5 | East St. Louis (Senior) HS | A | Bell | Box | Glenn | Hawkins | 4:16.10 | Unverified: |
